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Isolation of Cancer Stem Cells From Human Prostate Cancer Samples
Published on: March 14, 2014
14.6K
Andrographolide induces DNA damage in prostate cancer cells.
Ingrid S Forestier-Román1,2, Andrés López-Rivas2,3, María M Sánchez-Vázquez2
1Department of Biochemistry, School of Medicine, University of Puerto Rico, San Juan, Puerto Rico, USA.
Oncotarget
|February 26, 2019
Summary
Andrographolide effectively inhibits prostate cancer (PCa) by inducing DNA damage in both cell cultures and animal models. This natural compound reduces tumor growth, migration, and invasion, offering a promising therapeutic avenue.
Area of Science:
- Oncology
- Pharmacology
- Molecular Biology
Background:
- Prostate cancer (PCa) is a leading cause of cancer mortality in men.
- Andrographolide, derived from Andrographis paniculata, exhibits known anticancer properties.
Purpose of the Study:
- To investigate the efficacy of Andrographolide in prostate cancer models.
- To elucidate the molecular mechanisms underlying Andrographolide's action in PCa.
Main Methods:
- In vitro studies using androgen-independent (PC3) and androgen-dependent (22RV1) PCa cell lines.
- In vivo orthotopic xenograft model in SCID mice using 22RV1 cells.
- Analysis of cell motility, proliferation, apoptosis, tumor volume, gene expression, and DNA damage markers (γH2AX).
Main Results:
- Andrographolide reduced PCa cell migration, invasion, and increased apoptosis in vitro.
- In vivo, Andrographolide decreased tumor volume, MMP11 expression, and angiogenesis.
- Gene expression analysis revealed alterations in cellular compromise, cell cycle, and DNA repair pathways, with increased DNA double-strand break repair markers.
Conclusions:
- Andrographolide demonstrates significant anti-prostate cancer activity.
- The compound inhibits PCa progression by inducing DNA damage and associated repair mechanisms.
- Andrographolide presents a potential therapeutic agent for prostate cancer treatment.
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