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Gelatin Methacryloyl Granular Hydrogel Scaffolds: High-throughput Microgel Fabrication, Lyophilization, Chemical Assembly, and 3D Bioprinting
Published on: December 9, 2022
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Stability improvement and characterization of bioprinted pectin-based scaffold
Samuel Stealey1, Xiaoru Guo2, Lixia Ren3
11 Physics and Chemistry Department, Milwaukee School of Engineering, Milwaukee, WI, USA.
Journal of Applied Biomaterials & Functional Materials
|February 27, 2019
Summary
Chitosan modification enhances bioprinted scaffold stability, with 150 kD chitosan showing the most significant improvement. Oligochitosan was ineffective as a cross-linker for pectin-based bio-inks.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Bioprinting offers a promising alternative for creating tissues and organs for transplantation.
- Improving the stability of bioprinted scaffolds is crucial for their clinical application.
Purpose of the Study:
- To investigate the efficacy of oligochitosan as a novel cross-linker for pectin-based bio-inks.
- To evaluate the impact of post-printing modification using chitosan on the stability of bioprinted scaffolds.
Main Methods:
- Oligochitosan was tested as a cross-linker for pectin-based bio-ink.
- Bioprinted scaffolds were modified post-printing with chitosan of varying molecular weights.
- Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used for characterization.
Main Results:
- Oligochitosan did not function as a viable cross-linker for the pectin-based bio-ink.
- FTIR and SEM analyses confirmed successful post-printing modification of the scaffolds with chitosan.
- Chitosan-modified scaffolds demonstrated improved stability compared to unmodified scaffolds.
Conclusions:
- Post-printing modification with chitosan significantly enhances the stability of bioprinted scaffolds.
- Scaffolds modified with 150 kD chitosan exhibited the highest degree of stability.
- Oligochitosan is not suitable as a direct cross-linker for this pectin-based bio-ink system.
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