Related Experiment Video
Updated: Jan 28, 2026

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
Published on: September 15, 2016
Validation of Microarray for the Simultaneous Detection of Common α- and β-Thalassemia Gene Mutations
Sakorn Pornprasert1, Rinradee Anurak1, Chedtapak Ruengdit1
1Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand.
Background:
Methods for detecting the complex genetic characteristics of α- and β-thalassemias are required for preventing and controlling the outbreak of new cases.
Methods:
We evaluated the accuracy and practical utility of microarray for simultaneous detection of α- and β-thalassemias. A total of 102 DNA specimens, which represented 25 different genotypes, were tested in parallel using the microarray and reference methods used in the thalassemia laboratory of the Associated Medical Sciences-Clinical Services Center (AMS-CSC), Chiang Mai, Thailand.
Results:
A total of 100 (98.0%) DNA specimens were completely concordant between the microarray and reference methods, whereas discrepancies between the different methods were observed in only 2 DNA specimens with homozygous hemoglobin E (HbE).
Conclusions:
The microarray appeared to be a fast, easy to perform, and accurate method for simultaneous detection of α- and β-thalassemias in Thailand and Southeast Asian countries. However, this technique needs to be improved and validated in a larger number of specimens with homozygous HbE before further routine laboratory use.
Related Concept Videos
Mutation, Gene Flow, and Genetic Drift
Cell Specific Gene Expression
Conjugate Addition to α,β-Unsaturated Carbonyl Compounds
Mutations
Common Ion Effect
Reliability and Validity

