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Updated: Jan 28, 2026

Bergmeyer Glucose Quantification for Microbiological Samples
Published on: January 17, 2025
Glucose Oxidase-Instructed Fluorescence Amplification Strategy for Intracellular Glucose Detection
Shanshan Jiang1, Yifan Zhang1, Yichen Yang1
1Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, International Cancer Center, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering , Shenzhen University Health Science Center , Shenzhen 518060 , China.
Abstract:
The accurate detection of glucose at cellular level remains a big challenge. In this study, a signal amplification strategy mediated by silver nanocube (AgNC), glucose oxidase (GOx), and silver ion fluorescence probe (denoted as AgNC-GOx/Ag+-FP) is proposed for amplified intracellular glucose detection. The AgNC is oxidized into Ag+ by H2O2 generated from GOx-catalyzed glucose oxidation reaction, and Ag+ remarkably enhances the red fluorescence of Ag+-FP. Our results show that AgNC-GOx/Ag+-FP is highly sensitive and specific to glucose and H2O2. Afterward, the feasibility of using AgNC-GOx/Ag+-FP to detect intracellular glucose is verified in five different cell lines. In summary, a sensitive and specific fluorescence amplification strategy has been developed for intracellular glucose detection.
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