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Updated: Jan 28, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Fluorescence lifetime imaging of intracellular magnesium content in live cells
Azzurra Sargenti1, Alessia Candeo2, Giovanna Farruggia3
1Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy. stefano.iotti@unibo.it.
Abstract:
The first detailed analysis of FLIM applications for Mg cell imaging is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence lifetime imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.
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