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Dioscin Attenuates Myocardial Damages in Diabetic Rats maybe by Regulating NO-sGC-cGMP-PKG Pathway

Qian Wei1, Tonggang Zhu2, Xue Xiao1

  • 1Department of Heart Disease, Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin, China.

Annals of Clinical and Laboratory Science
|March 1, 2019
PubMed
Abstract

Insights

Dioscin improves cardiac function and reduces inflammation in diabetic rats by enhancing the NO-sGC-cGMP-PKG pathway, offering a potential treatment for diabetic cardiomyopathy.

Area of Science:

  • Cardiovascular Research
  • Diabetology
  • Pharmacology

Background:

  • Diabetic cardiomyopathy is a significant complication of diabetes mellitus.
  • Streptozotocin (STZ)-induced diabetes in rats is a common model for studying diabetic complications.
  • Understanding the mechanisms of myocardial injury in diabetes is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the protective effects of dioscin on the myocardium of STZ-induced diabetic rats.
  • To elucidate the underlying molecular mechanisms, particularly focusing on the NO-sGC-cGMP-PKG pathway.

Main Methods:

  • Establishment of a rat model of diabetes using streptozotocin (STZ).
  • Administration of varying doses of dioscin (100 and 200 μg/kg/day) to diabetic rats for 6 weeks.
  • Assessment of hemodynamic parameters, myocardial histology (HE staining), and protein expression (Western blot) related to inflammation and the NO-sGC-cGMP-PKG pathway.

Main Results:

  • Dioscin treatment improved left ventricular function, evidenced by increased ejection fraction (EF) and decreased LVEDP and Tau.
  • Histopathological examination revealed that dioscin ameliorated myocardial lesions in diabetic rats.
  • Dioscin significantly reduced levels of inflammatory cytokines (TGF-β1, TNF-α, IL-1β) and modulated the NO-sGC-cGMP-PKG pathway by affecting PDE-5, PKG, and p-VASP levels.

Conclusions:

  • Dioscin demonstrates cardioprotective effects in STZ-induced diabetic rats.
  • The mechanism involves the upregulation of the nitric oxide-soluble guanylate cyclase-cyclic guanosine monophosphate-protein kinase G (NO-sGC-cGMP-PKG) pathway.
  • Dioscin holds potential as a therapeutic agent for diabetic cardiomyopathy.
Keywords:
DioscinNO-sGC-cGMP-PKG pathwaydiabetic cardiomyopathymyocardial inflamation

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