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Updated: Jan 28, 2026

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
Published on: April 5, 2017
Sildenafil does not affect the proliferation of human lymphocytes in the in vitro transplant model
Beata Kaleta1, Agnieszka Boguska1
1Department of Clinical Immunology, Transplantation Institute, Medical University of Warsaw, Warsaw, Poland.
Abstract:
Sildenafil is used in the treatment of erectile dysfunction and pulmonary arterial hypertension. Numerous studies revealed beneficial effects of its use in renal, liver, heart and bone marrow transplant recipients. Some reports suggested that the drug modulates the function of the immune system, however, its influence on antigen-induced proliferation of lymphocytes remains unknown. Thus, the aim of the study was to investigate the effects of sildenafil on human peripheral blood mononuclear cells (PBMCs) proliferation in a mixed lymphocyte reaction. It was demonstrated that the drug did not affect auto- and alloantigen-induced proliferation of PBMCs and showed no cytotoxic effect.
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