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DNA sequence of the murine gamma 1 switch segment reveals novel structural elements
Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1986
Summary
The immunoglobulin heavy chain switch regions, crucial for B lymphocyte class switching, show complex structures. The S gamma 1 segment contains tandem repeats and unique direct repeat elements, varying in length across different Igh haplotypes.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Immunoglobulin heavy chain switch regions are vital DNA segments for B lymphocyte class switching.
- These segments exhibit structural organization based on tandemly repeated unit sequences, varying in length across murine isotypes.
Purpose of the Study:
- To determine the complete nucleotide sequence of the cloned S gamma 1 segment from BALB/c DNA.
- To characterize the sequence elements and structural organization of the S gamma 1 segment.
Main Methods:
- DNA sequencing of the cloned S gamma 1 segment.
- Southern hybridization experiments to analyze different Igh haplotypes.
Main Results:
- The S gamma 1 segment comprises over 120 tandemly repeated 49mers.
- Unique sequences identified as direct repeat elements (DR II and DR I) are integrated within the S gamma 1 structure.
- The basic structural scheme of S gamma 1 is conserved across eight Igh haplotypes, with variations in the number of 49mer elements.
Conclusions:
- The S gamma 1 segment possesses a complex structure involving tandem repeats and direct repeat elements.
- Structural variations in S gamma 1 exist across different Igh haplotypes, primarily in the number of repeat units.
- S gamma 1, S gamma 3, and S gamma 2b segments represent distinct sets of tandemly repeated sequences.