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Processing serial crystallography data with CrystFEL: a step-by-step guide.

Thomas A White1

  • 1Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, Hamburg, Germany.

Acta Crystallographica. Section D, Structural Biology
|March 2, 2019
PubMed
Summary
This summary is machine-generated.

This guide details using CrystFEL software for processing serial crystallography data from X-ray free-electron lasers and synchrotrons. It offers practical, user-focused steps and command-line examples for data analysis.

Keywords:
CrystFELX-ray free-electron lasersdata processingserial crystallography

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Area of Science:

  • Structural Biology
  • Biophysics
  • Crystallography

Background:

  • Serial crystallography is a powerful technique for determining protein structures.
  • Processing data from X-ray free-electron lasers (XFELs) and synchrotrons presents unique challenges.
  • Existing literature often focuses on theory rather than practical application.

Purpose of the Study:

  • To provide a user-friendly, step-by-step guide for processing serial crystallography data.
  • To illustrate the practical application of the CrystFEL software package.
  • To assist researchers in analyzing data from XFEL and synchrotron sources.

Main Methods:

  • Detailed explanation of the CrystFEL software workflow.
  • Inclusion of command-line examples for each processing step.
  • Focus on a user-centric approach to data analysis.

Main Results:

  • A comprehensive, practical guide to serial crystallography data processing.
  • Demonstration of CrystFEL's utility for diverse experimental setups.
  • Facilitation of reproducible data analysis through clear instructions.

Conclusions:

  • CrystFEL is an effective tool for serial crystallography data processing.
  • This guide empowers users to confidently analyze their experimental data.
  • The practical approach enhances accessibility to advanced structural biology techniques.