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Simple Method for Anaerobic Inoculation of Foods in Cans, Retort Pouches, Glass Jars or Semi-Rigid Containers
1Food Directorate, Bureau of Microbial Hazards, Health Protection Branch, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2.
Abstract:
Cured silicon sealant (SS) on lids of cans and glass jars, on retort pouch and semi-rigid container surfaces was employed for providing a port of entry during inoculation of foods while maintaining anaerobiosis. Following inoculation, the SS was covered by molten wax and aluminum foil. Anaerobic growth in the packs was demonstrated by gas formation by several species of clostridia. Staphylococci also grew anaerobically.
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