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Updated: Jan 28, 2026

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
Published on: October 25, 2018
Control of Foaming during Microbiological Analysis of Foods and Recovery of Indicator Organisms
A K Stersky1, G W Riedel1, C Thacker1
1Food Directorate, Bureau of Microbial Hazards, Health Protection Branch, Health and Welfare Canada, Tunney's Pasture, Ottawa, Ontario, Canada K1A 0L2 and National Microbiological Monitoring Laboratory, Field Operations Directorate, Health Protection Branch, Health and Welfare Canada, Halifax, Nova Scotia, Canada B3J 2R7.
Abstract:
Persistent foam formation occurring during blending of jelly powder (JP), whole egg powder (WEP) and skim milk power (SMP) was demonstrated. Reduction of foaming was uniformly best achieved by using the Colworth Stomacher 400 (STOM) for mixing or blending under a vacuum (VAC). Two hundred, 1000 and 6000 ppm of Dow Corning Antifoam Agent (DAF) was necessary to reduce foaming of JP, SMP and WEP, respectively. Escherichia coli was recovered in similar numbers from JP, SMP and WEP by normal blending (N), STOM, VAC or by the application of 200 ppm of DAF. Significantly lower recovery of Staphylococcus aureus from JP resulted when N or VAC was employed and from SMP when STOM was used. Streptococcus faecalis showed significantly lower recoveries from JP and WEP when STOM or VAC and DAF at 200 ppm were used.
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