Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A medium-term corneal preserving medium (K-Sol).

C W Yau, H E Kaufman

Archives of Ophthalmology (Chicago, Ill. : 1960)

|April 1, 1986

Summary

K-Sol, a new corneal preserving solution, effectively preserved rabbit corneas for 12 days. The 2.5% chondroitin sulfate concentration demonstrated optimal results for donor tissue in penetrating keratoplasty.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of topical unoprostone and latanoprost on acute and recurrent herpetic keratitis in the rabbit.

American journal of ophthalmology·2001

Same author

Increased severity of HSV-1 keratitis and mortality in mice lacking the 2-5A-dependent RNase L gene.

Investigative ophthalmology & visual science·2001

Same author

Treatment of viral diseases of the cornea and external eye.

Progress in retinal and eye research·1999

Same author

HSV-1 migration in latently infected and naive rabbits after penetrating keratoplasty.

Investigative ophthalmology & visual science·1999

Same author

Reactivation of herpes virus after lamellar keratoplasty.

Japanese journal of ophthalmology·1999

Same author

Confocal microscopy in lattice corneal dystrophy.

Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie·1999

Area of Science:

Ophthalmology
Biomaterials Science
Tissue Engineering

Background:

Corneal transplantation, or penetrating keratoplasty, relies on effective donor tissue preservation.
Current preservation solutions face limitations in medium-term storage efficacy.
Development of novel preservation media is crucial for improving graft survival rates.

Purpose of the Study:

To evaluate the efficacy of a novel corneal preserving solution, K-Sol, for medium-term storage of donor corneal tissue.
To determine the optimal concentration of chondroitin sulfate in K-Sol for preserving corneal tissue.
To assess the viability and suitability of K-Sol preserved corneas for penetrating keratoplasty.

Main Methods:

K-Sol was formulated with TC 199, gentamicin sulfate, HEPES buffer, and varying chondroitin sulfate concentrations (2.0%, 2.25%, 2.5%).
Rabbit corneas were stored at 4°C for 12 days in K-Sol solutions and compared to fresh controls.
Preserved corneas were used for penetrating keratoplasty, with graft outcomes assessed three weeks post-surgery, including clarity, thickening, pachymetry, and endothelial cell loss.

Main Results:

Corneas preserved in K-Sol, particularly with 2.5% chondroitin sulfate, remained clear with minimal thickening after 12 days of storage.
Graft survival and clarity were high across most K-Sol concentrations, with only one graft opacifying in the 2.0% group.
No significant differences in pachymetry or endothelial cell loss were observed between K-Sol preserved grafts and fresh controls.

Conclusions:

K-Sol, especially at a 2.5% chondroitin sulfate concentration, represents a simple and effective medium-term corneal preservation solution.
This formulation supports the viability of donor tissue for penetrating keratoplasty in rabbit models.
K-Sol shows promise for enhancing the availability and success of corneal transplantation.

Related Experiment Videos