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Acoustofluidic particle steering.

Zaid Shaglwf1, Bjorn Hammarström2, Dina Shona Laila3

  • 1School of Engineering, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom.

The Journal of the Acoustical Society of America
|March 3, 2019
PubMed
Summary

This study introduces an active acoustic manipulation system that precisely controls micro-objects. It uses a feedback loop and resonance frequencies for accurate steering, overcoming limitations of fixed force fields.

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Area of Science:

  • Acoustic manipulation
  • Microfluidics
  • Biophysics

Background:

  • Acoustic radiation forces offer precise micro-object manipulation.
  • Traditional methods face challenges with fixed force distributions and a priori prediction difficulties.

Purpose of the Study:

  • To develop an active feedback-controlled system for steering micro-objects using acoustic radiation forces.
  • To overcome limitations of fixed force distributions and unpredictable acoustic fields in microfluidic devices.

Main Methods:

  • Utilized a computer-controlled feedback loop with MATLAB and a microscope camera for particle imaging.
  • Employed a planar resonator for axial levitation and algorithmic selection of a second resonance frequency for lateral manipulation.
  • Leveraged gradients in kinetic energy density of the acoustic field for precise force generation.

Main Results:

  • Demonstrated manipulation of 10 μm microbeads over distances exceeding 100s of micrometers.
  • Achieved manipulation paths of 200 μm length within manipulation times of approximately 10 seconds.
  • Successfully controlled micro-objects without requiring prior knowledge of the acoustic force field structure.

Conclusions:

  • The developed active approach enables precise and predictable steering of micro-objects using acoustic forces.
  • This method overcomes key challenges in acoustic manipulation, offering a versatile tool for microfluidic applications.
  • The system's ability to adapt and control forces in real-time opens new possibilities for micro-object handling.