Related Experiment Video
Updated: Jan 23, 2026

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
Published on: October 31, 2025
Analysis of UDP-Sugars from Cultured Cells and Small Tissue Samples
Sanna Oikari1,2, Markku I Tammi3
1Institute of Biomedicine, University of Eastern Finland, Kuopio, Finland. sanna.oikari@uef.fi.
Abstract:
UDP-sugars are important substrates for the synthesis of various cellular glycans and glycoconjugates, many of which play essential roles in the pathobiology of diseases associated with deranged glucose metabolism, such as cancer and type 2 diabetes. Hence, their analysis from cultured cells and especially from tissue samples can give valuable information. This chapter describes a method for UDP-sugar isolation from various sources utilizing ion-pair solid-phase extraction with graphitized carbon cartridges, and their analysis using anion-exchange high-performance liquid chromatography.
Related Concept Videos
Phloem and Sugar Transport
Plant Tissue Culture
Plant Cells and Tissues
Sugars as Energy Storage Molecules
Sample Preparation for Analysis: Overview
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
Sample Preparation for Analysis: Advanced Techniques
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...

