Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Low molecular weight IgM. Detection using immunoblotting.

R Harries, I Beckman, P Roberts-Thomson

    Journal of Immunological Methods
    |April 3, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Is there a role for botulinum toxin A in the emergency setting for delayed abdominal wall closure in the management of the open abdomen? A systematic review.

    Annals of the Royal College of Surgeons of England·2022
    Same author

    Variations in competencies needed to complete surgical training.

    BJS open·2019
    Same author

    Testicular torsion treatment: the horns of a dilemma?

    Annals of the Royal College of Surgeons of England·2019
    Same author

    Modular emergency general surgery training: A pilot study of a novel programme.

    Annals of the Royal College of Surgeons of England·2016
    Same author

    Monoclonal antibodies to HLA-DR antigens.

    Immunology today·2014
    Same author

    Spontaneous oesophageal perforations in a competitive swimmer.

    Journal of surgical case reports·2014
    Same journal

    Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

    Journal of immunological methods·2026
    Same journal

    Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

    Journal of immunological methods·2026
    Same journal

    Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

    Journal of immunological methods·2026
    Same journal

    Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

    Journal of immunological methods·2026
    Same journal

    Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

    Journal of immunological methods·2026
    Same journal

    Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

    Journal of immunological methods·2026
    See all related articles

    A modified immunoblotting assay is a more sensitive and reliable method for identifying low molecular weight (LMW) IgM. This technique can detect additional IgM forms, aiding in the diagnosis of polymerization disorders.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Low molecular weight (LMW) Immunoglobulin M (IgM) is a clinically significant biomarker.
    • Accurate identification of LMW IgM is crucial for prognostic assessment in various disorders.

    Purpose of the Study:

    • To compare the efficacy of two distinct techniques for identifying LMW IgM.
    • To evaluate the sensitivity and reliability of filtration chromatography/nephelometry versus a modified immunoblotting assay.

    Main Methods:

    • Comparative analysis of filtration chromatography with nephelometry against a modified immunoblotting assay.
    • Assessment of diagnostic resolution and sensitivity for LMW IgM detection.

    Main Results:

    • The modified immunoblotting assay demonstrated superior sensitivity and reliability compared to filtration chromatography/nephelometry.

    Related Experiment Videos

  • Immunoblotting effectively resolved monomeric and additional oligomeric forms of IgM, indicating polymerization abnormalities.

    • Conclusions:

    • The modified immunoblotting assay is a highly recommended method for detecting LMW IgM.
    • This technique's ability to identify diverse IgM oligomers suggests its utility as a screening tool for polymerization disorders with prognostic implications.