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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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A multi-mode digital holographic microscope.

James L Flewellen1, Irwin M Zaid2, Richard M Berry2

  • 1Immune Receptor Activation Laboratory, The Francis Crick Institute, London NW1 1AT, United Kingdom.

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|March 6, 2019
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Summary
This summary is machine-generated.

This study introduces a versatile digital holographic microscope with three imaging modes for tracking microorganisms and nanoparticles. It enables precise measurements of sample properties like refractive index and dry mass.

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Area of Science:

  • Microscopy
  • Optical Physics
  • Biophysics

Background:

  • Conventional microscopy struggles with imaging certain microscopic objects and quantifying their properties.
  • Digital holographic microscopy offers advanced capabilities but often lacks versatility.
  • A need exists for a unified platform combining multiple imaging modalities for comprehensive sample analysis.

Purpose of the Study:

  • To develop and demonstrate a transmission-mode digital holographic microscope with switchable inline, dark field off-axis, and bright field off-axis imaging.
  • To showcase the instrument's ability to track motile microorganisms in 3D, localize nanoparticles, and perform quantitative phase recovery.
  • To validate the microscope's performance across different imaging modes and applications.

Main Methods:

  • A novel transmission-mode digital holographic microscope was designed with three distinct imaging configurations.
  • Inline mode utilized high-speed video recording (up to 2000 fps) for 3D tracking of dielectric objects.
  • Off-axis modes employed a physical mask for high-magnification dark field imaging of nanoparticles and quantitative phase recovery for refractive index and dry mass measurements.

Main Results:

  • Successfully tracked motile bacteria in 3D at 45× magnification.
  • Localized 100 nm gold nanoparticles using dark field off-axis holography at 225× magnification up to 16 μm.
  • Measured refractive index of a test target and dry mass of human erythrocytes using bright field off-axis quantitative phase microscopy.

Conclusions:

  • The developed digital holographic microscope offers a versatile and powerful platform for diverse microscopic investigations.
  • The instrument's multi-modal capability enhances the study of dynamic biological processes and material characterization.
  • This technology advances high-resolution imaging and quantitative analysis of microscopic samples.