Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microvascular metabolism in diabetes.

T S Kern, R L Engerman

    Metabolism: Clinical and Experimental
    |April 1, 1986
    PubMed
    Summary

    High blood sugar (hyperglycemia) may contribute to diabetic microvascular disease. Canine retinal and cerebral microvessels produce galactitol, an effect inhibited by aldose reductase inhibitors, suggesting a role for polyol production.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Omega-3 polyunsaturated fatty acids preserve retinal function in type 2 diabetic mice.

    Nutrition & diabetes·2013
    Same author

    Long-term type 1 diabetes influences haematopoietic stem cells by reducing vascular repair potential and increasing inflammatory monocyte generation in a murine model.

    Diabetologia·2012
    Same author

    NF-κB subunits are differentially distributed in cells of lumbar dorsal root ganglia in naïve and diabetic rats.

    Neuroscience letters·2010
    Same author

    Validation of structural and functional lesions of diabetic retinopathy in mice.

    Molecular vision·2010
    Same author

    PP2A contributes to endothelial death in high glucose: inhibition by benfotiamine.

    American journal of physiology. Regulatory, integrative and comparative physiology·2010
    Same author

    Comparison of three strains of diabetic rats with respect to the rate at which retinopathy and tactile allodynia develop.

    Molecular vision·2010

    Area of Science:

    • Biochemistry
    • Vascular Biology
    • Diabetic Complications

    Background:

    • Hyperglycemia is linked to diabetic retinal vascular disease.
    • Understanding hexose metabolism in vascular cells is crucial.
    • Microvessels metabolize hexoses independently of insulin.

    Purpose of the Study:

    • To investigate hexitol production in retinal and cerebral microvessels.
    • To explore the role of polyol accumulation in diabetic microvascular disease.
    • To assess the effect of aldose reductase inhibition on microvessel hexitol production.

    Main Methods:

    • Isolation of metabolically active retinal and cerebral microvessels from dogs.
    • Quantification of hexitol-producing metabolic activity.
    • Perfusion to remove erythrocytes prior to microvessel isolation.
    • Assessment of sorbinil's effect on galactitol production.

    Main Results:

    • Microvessels metabolize glucose via glycolysis, pentose phosphate shunt, and glycogenogenesis.
    • Diabetic animal microvessels show subnormal glucose metabolism.
    • Retinal and cerebral microvessels produce galactitol from galactose.
    • Sorbinil significantly inhibits galactitol production in microvessels.

    Conclusions:

    • Microvessels possess hexitol-producing metabolic activity.
    • Polyol production may contribute to the etiology of diabetic microvascular disease.
    • Aldose reductase inhibition shows potential in mitigating microvascular complications.

    Related Experiment Videos