Metal Ions in Activated Carbon Improve the Detection Efficiency of Aflatoxin-Producing Fungi
Tadahiro Suzuki1, Masatoshi Toyoda2
1Division of Food Biotechnology, Food Research Institute, NARO, 2-1-12 Kannon-dai, Tsukuba, Ibaraki 305-8642, Japan. suzut@affrc.go.jp.
Abstract:
Aflatoxins (AF), produced by several Aspergillus species, are visible under ultraviolet light if present in high amounts. AF detection can be improved by adding activated carbon, which enhances the observation efficiency of weakly AF-producing fungi. However, commercial activated carbon products differ in their characteristics, making it necessary to investigate which characteristics affect method reproducibility. Herein, the addition of 10 activated carbon products resulted in different AF production rates in each case. The differences in the production of aflatoxin G₁ (AFG₁) were roughly correlated to the observation efficiency in the plate culture. Trace element analysis showed that the concentrations of several metal ions differed by factors of >100, and the carbons that most effectively increased AFG₁ production contained higher amounts of metal ions. Adding 5 mg L-1 Fe or Mg ions increased AFG₁ production even without activated carbon. Furthermore, co-addition of both ions increased AFG₁ production stably with the addition of carbon. When varying the concentration of additives, only AFG₁ production increased in a concentration-dependent manner, while the production of all the other AFs decreased or remained unchanged. These findings suggest that a key factor influencing AF production is the concentration of several metal ions in activated carbon and that increasing AFG₁ production improves AF detectability.
Related Concept Videos
The Roles of Bacteria and Fungi in Plant Nutrition
Alkali Metals
Table 1: Properties of the alkali metals
Ions as Acids and Bases
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
Bonding in Metals
The Carbon Cycle
Metal-Ligand Bonds
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...


