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Microbial Colonization Coordinates the Pathogenesis of a Klebsiella pneumoniae Infant Isolate

Jillian L Pope1, Ye Yang1, Rachel C Newsome1

  • 1Department of Medicine, University of Florida, Gainesville, Florida, USA.

Scientific Reports
|March 6, 2019
PubMed

Insights

Klebsiella pneumoniae from infant stool can cause inflammation and DNA damage. Its pathogenicity is influenced by gut microbiota composition, highlighting the importance of early colonization patterns.

Area of Science:

  • Microbiology
  • Gastroenterology
  • Immunology

Background:

  • Enterobacteriaceae, including Klebsiella pneumoniae, are early gut colonizers in neonates.
  • Some pathobionts, like K. pneumoniae, can cause disease under specific conditions.
  • The interaction between developing gut microbiota and pathobiont virulence is not well understood.

Purpose of the Study:

  • To investigate the functional interaction between K. pneumoniae colonization patterns and host response.
  • To determine the pathogenic potential of an infant-derived K. pneumoniae isolate (51-5) carrying the pks genotoxin island.
  • To assess the impact of microbiota on K. pneumoniae-induced inflammation and tumor development.

Main Methods:

  • In vitro studies using intestinal epithelial cells, macrophages, and splenocytes.
  • Gnotobiotic experiments in Il10 knockout mice to assess in vivo inflammation.
  • Tumorigenesis studies in ApcMin/+; Il10 knockout mice.
  • Analysis of proinflammatory gene expression, DNA damage, and cell cycle arrest.

Main Results:

  • K. pneumoniae 51-5 upregulated proinflammatory genes in vitro and induced intestinal inflammation in vivo.
  • The isolate accelerated inflammation onset in Il10 knockout mice, especially with naturally acquired microbiota.
  • K. pneumoniae 51-5 induced DNA damage and cell cycle arrest.
  • Tumor development in ApcMin/+; Il10 knockout mice was not significantly affected by the absence of the colibactin-activating enzyme ClbP.

Conclusions:

  • Pathogenicity of the infant K. pneumoniae isolate is sensitive to microbial colonization status.
  • Early colonization by pathobionts like K. pneumoniae can have significant health implications.
  • Further research is needed to understand the complex interplay between microbiota and pathobiont virulence in neonates.

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