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Related Experiment Video

Updated: Jan 28, 2026

Culture of Isolated Floor Plate Tissue and Production of Conditioned Medium to Assess Functional Properties of Floor Plate-released Signals
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Polymer-based controlled-release fed-batch microtiter plate - diminishing the gap between early process development

T Keil1, B Dittrich2, C Lattermann3

  • 11AVT - Biochemical Engineering, RWTH Aachen University, Forckenbeckstraße 51, 52074 Aachen, Germany.

Journal of Biological Engineering
|March 6, 2019
PubMed
Summary

This study introduces a cost-effective polymer-based fed-batch system in microtiter plates, enabling controlled glucose release for microbial cultivations. This small-scale system enhances product formation significantly compared to traditional batch methods.

Keywords:
Bioprocess developmentEscherichia coliFed-batchHansenula polymorphaHigh-throughputMicrotiter plateScreening

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Microbial Cultivation

Background:

  • Industrial fed-batch cultivations prevent issues like overflow metabolites and oxygen limitation common in batch cultures.
  • Small-scale bioprocess development typically uses batch operations, contrasting with industrial fed-batch methods.
  • Existing small-scale fed-batch systems are often costly and complex.

Purpose of the Study:

  • To investigate a cost-efficient polymer-based fed-batch system for controlled substrate release in microtiter plates.
  • To mimic industrial fed-batch conditions at a small scale for improved bioprocess development.
  • To enhance throughput and reduce costs in early-stage bioprocess optimization.

Main Methods:

  • Immobilization of a silicone matrix with embedded glucose crystals in microtiter plate wells.
  • Cultivation of model organisms (E. coli, H. polymorpha) under controlled fed-batch conditions.
  • Development of a mathematical tool to predict glucose release rates based on media conditions.

Main Results:

  • Glucose release rate is influenced by osmotic concentration, pH, temperature, and nitrogen source.
  • Fed-batch conditions in microtiter plates were comparable to large-scale cultivations, confirmed by online and offline monitoring.
  • Product formation was enhanced by a factor of 245 compared to batch cultivations.

Conclusions:

  • The polymer-based fed-batch microtiter plate system effectively mimics industrial fed-batch conditions at a small scale.
  • This system enables more reliable strain screening and cost-efficient early process development.
  • A systematic and economical scale-down approach is achievable with this technology.