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Allotypic differences in murine mu genes.

P H Schreier, S Quester, A Bothwell

    Nucleic Acids Research
    |March 11, 1986
    PubMed
    Summary
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    Researchers sequenced the C57BL/6 mouse heavy chain mu b allele DNA. This reveals high homology with other mouse IgM alleles, with a single amino acid change defining the mu b allotype.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Comparative Genomics

    Background:

    • The immunoglobulin heavy chain mu (IgM) is crucial for B cell development and immune responses.
    • Allelic variations in immunoglobulin genes contribute to immune system diversity and function.
    • Previous studies identified significant differences between gamma 2a immunoglobulin heavy chain alleles.

    Purpose of the Study:

    • To determine the complete DNA sequence of the C57BL/6 mouse heavy chain mu b allele.
    • To compare this sequence with other known mouse IgM alleles (mu a).
    • To identify the molecular basis of the mu b allotypic determinant.

    Main Methods:

    • cDNA cloning and complete DNA sequencing of the C57BL/6 mouse heavy chain mu b allele.
    • Comparative sequence analysis against germ line BALB/c mu a and TEPC-183 mu a alleles.

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  • Identification of nucleotide differences and their impact on amino acid sequences.

    • Main Results:

    • A high degree of nucleotide sequence homology was observed between the C57BL/6 mu b and BALB/c mu a alleles.
    • Only one nucleotide difference resulted in an amino acid substitution between the compared mu alleles.
    • Analysis of four distinct DNA sequences confirmed they represent different IgM alleles.

    Conclusions:

    • The C57BL/6 mu b and BALB/c mu a alleles are highly homologous.
    • A single amino acid substitution likely defines the allotypic determinant of the mu b allele.
    • The findings contribute to understanding the genetic basis of immunoglobulin diversity in mice.