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Updated: Jan 28, 2026

Detection and Removal of Tooth-Colored Composite Resin Using the Fluorescence-Aided Identification Technique
Published on: July 27, 2022
Fluorescence properties of demineralized enamel after resin infiltration and dental bleaching
Carlos Rocha Gomes Torres1, Rayssa Ferreira Zanatta2, Beatriz Maria Fonseca2
1Department of Restorative Dentistry, Institute of Science and Technology, São Paulo State University-UNESP, São Paulo, Brazil, carlos.rg.torres@unesp.br.
Purpose:
To evaluate the effects of different white spot lesion (WSL) treatments associated with dental bleaching on the fluorescence of dental enamel.
Methods:
80 flat enamel disks (3 mm diameter and 1 mm thick) were obtained from bovine incisors. The initial fluorescence (fluorescent emission or Delta E*ab- FL units) of the specimens was measured using a spectrophotometer. Artificial caries was created in all specimens, and the measurements were repeated. The specimens were divided into four groups according to the treatment applied (n = 20): CON (control) - immersion in ultrapure water for 8 weeks; SAL - immersion in artificial saliva for 8 weeks; FL - daily application of 0.05% sodium fluoride for 1 minute/artificial saliva for 8 weeks; and ICON - resin infiltration (Icon). After the treatments, the assessments were repeated. Dental bleaching using 35% hydrogen peroxide gel was performed on all specimens for 30 minutes, and the measurements were made again after 7 days. Data were submitted to ANOVA and Tukey tests across the treatments for each moment of evaluation.
Results:
Fluoride and saliva remineralization were not able to change enamel fluorescence, even after bleaching. Only resin infiltration increased the enamel fluorescence; however, after bleaching, all groups presented similar values. Icon increased translucency immediately after application, but bleaching reduced it to its initial values.
Clinical Significance:
Changes of fluorescence in infiltrated enamel might lead to unsatisfactory esthetics under certain conditions such as ultraviolet light.
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