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Updated: Jan 28, 2026

Author Spotlight: Process Development for the Spray-Drying of Probiotic Bacteria and Evaluation of the Product Quality
Published on: April 7, 2023
Microencapsulation of an indigenous isolate of Bacillus thuringiensis by spray drying
Ardahan Eski1, Zihni Demirbağ1, İsmail Demir1
1a Faculty of Sciences, Department of Biology , Karadeniz Technical University , Trabzon , Turkey.
Abstract:
In this study, microencapsulation by spray drying was performed to protect spores and crystals of an indigenous isolate of Bacillus thuringiensis Se13 from environmental stress. The effects of wall material, inlet temperature, and outlet temperature on microencapsulation of Bt-Se13 were investigated using Taguchi's orthogonal array. The most suitable wall material determined as maltodextrin DE10. The optimum inlet and outlet temperatures of spray drier were determined as 160 °C and 70 °C, respectively. The number of viable spores, mean particle size, wetting time, percentage of suspensibility and moisture content of the product produced under optimum conditions were determined as 8.1 × 1011 cfu g-1, 13.462 µm, 25.22 s, 77.66% and 7.29%, respectively. As a result of efficiency studies on Spodoptera exigua in the laboratory conditions, the LC50 was determined as 1.6 × 104 cfu mL-1. Microencapsulated Bt-Se13 based bio-pesticide may be registered for the control of S. exigua and can be tested against other lepidopterans which share the same environment.
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