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Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Minimizing Salmonella Contamination on Broiler Carcasses with Poly (Hexamethylenebiguanide Hydrochloride)
J E Thomson1, N A Cox1, J S Bailey1
1Richard B. Russell Agricultural Research Center, SEA, U.S. Department of Agriculture, P. O. Box 5677, Athens, Georgia 30613 and Department of Food Science and Human Nutrition, University of Delaware, Newark, Delaware 19711.
Abstract:
Broiler carcasses, each inoculated with 30 cells of marker Salmonella heidelberg , were prechilled and chilled together with uninoculated carcasses in a simulated commercial chilling system. When either 10 or 25 ppm of PHMB [poly(hexamethylenebiguanide hydrochloride)] was added to the prechill water, cross-contamination (uninoculated carcasses showing contamination with marker Salmonella after chilling) was prevented, and no viable Salmonella were found on the inoculated carcasses. When carcasses, each inoculated with 60,000 cells of marker Salmonella , were similarly chilled, and 10 ppm of PHMB was added to the prechill water, cross-contamination was not prevented, and viable Salmonella were found on the inoculated carcasses. With 60,000 cells, and 25 ppm PHMB, cross-contamination was prevented, but viable Salmonella remained on the inoculated carcasses.
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