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Updated: Jan 28, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
Published on: November 30, 2013
In Situ Hybridization in Mineralized Tissues: The Added Value of LNA Probes for RNA Detection
G Lignon1, D Hotton2, A Berdal1
1Molecular Oral Pathophysiology, Cordeliers Research Center, UMRS 1138 INSERM, Paris-Descartes, Pierre-et-Marie-Curie, Paris-Diderot Universities, Paris, France.
Abstract:
In situ hybridization (ISH) is one of the fundamental methods in developmental biology and neurobiology. Their first ISH protocols were reported in 1969 (Gall and Pardue, Proc Natl AcadSci USA 63:378-83, 1969). Since several decades, ISH based on the specific hybridization of 100-2000 nucleotides long probes enabled the localization of DNA/RNA sequences in tissues and cells with high cellular resolution. But sometimes a limited sensitivity notably in mineralized tissues (Obernosterer et al., Nature Protocols 2:1508-14, 2007).Here we describe a recent improvement of in situ hybridization efficiency by applying nucleotide locked nucleic acid (LNA)-incorporated oligodeoxynucleotide probes (20 LNA/DNA nucleotide probes) essentially used for noncoding miRNA and messenger RNAs.
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