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Updated: Jan 28, 2026
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In vitro Induction of Human Dental Pulp Stem Cells Toward Pancreatic Lineages
Published on: September 25, 2021
3.6K
A comparative study on different stemness gene expression between dental pulp stem cells vs. dental bud stem cells.
A Ballini1, S Cantore, S Scacco
1Department of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari Aldo Moro, Bari, Italy. Francesco.inchingolo@uniba.it.
European Review for Medical and Pharmacological Sciences
|March 7, 2019
Summary
Dental stem cells, including dental pulp stem cells (DPSCs) and stem cells from dental buds (DBSCs), exhibit mesenchymal stemness and osteogenic potential. These easily accessible cells show promise for regenerative medicine and tissue repair applications.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Dental Research
Background:
- Mesenchymal stem cells (MSCs) are crucial for regenerative medicine due to their differentiation capabilities.
- Dental tissues are an optimal source for adult stem cells, specifically dental pulp stem cells (DPSCs) and stem cells from dental buds (DBSCs).
- These dental stem cells can differentiate into various cell types, including osteoblasts, chondrocytes, and neurons.
Purpose of the Study:
- To comparatively analyze the stemness properties of DPSCs and DBSCs.
- To investigate the expression of key stemness genes (Nanog, OCT4, Sox2, c-Myc, Klf4) in dental stem cells.
- To evaluate the osteogenic potential of DPSCs and DBSCs for regenerative applications.
Main Methods:
- Collection of normal impacted third molars and tooth buds from adults and adolescents.
- Isolation and culture of dental pulp stem cells (DPSCs) and stem cells from dental buds (DBSCs).
- Quantitative reverse transcription PCR (qRT-PCR) to assess the expression of stemness genes in differentiated and undifferentiated cells.
Main Results:
- Both DPSCs and DBSCs are easily accessible and express typical mesenchymal stemness markers.
- The presence of embryonic gene regulators (Nanog, OCT4, Sox2, c-Myc, Klf4) confirms their stemness and osteogenic capacity.
- DPSCs and DBSCs demonstrate potential as valid tools for regenerative medicine and translational applications.
Conclusions:
- DPSCs and DBSCs possess distinct genetic traits related to their origin and maturation stage.
- These dental stem cells offer significant potential for clinical applications in autologous procedures.
- Applications include regeneration of bone, hard tissues, and soft tissues, with advantages of easy isolation, availability, biocompatibility, safety, and no ethical restrictions.
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