Related Experiment Video

Updated: Jan 28, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.7K

Reliable resolution of ambiguous hepatitis C virus genotype 1 results with the Abbott HCV Genotype Plus RUO assay

V Saludes1,2,3, A Antuori1, B Reinhardt4

  • 1Microbiology Department, Laboratori Clínic Metropolitana Nord, Hospital Universitari Germans Trias i Pujol; Genetics and Microbiology Department, Universitat Autònoma de Barcelona, Badalona, Spain.

Scientific Reports
|March 8, 2019
PubMed

Insights

Accurate hepatitis C virus genotype 1 (HCV-1) subtyping is crucial. The Abbott HCV Genotype Plus (GT Plus) assay effectively resolved ambiguous results, improving overall subtyping accuracy in clinical samples.

Area of Science:

  • Virology
  • Clinical Diagnostics
  • Molecular Biology

Background:

  • Accurate subtyping of hepatitis C virus genotype 1 (HCV-1) is essential for clinical and epidemiological purposes.
  • Existing methods can yield ambiguous results, necessitating improved diagnostic approaches.

Purpose of the Study:

  • To evaluate the Abbott HCV Genotype Plus (GT Plus) assay as a reflex test for resolving ambiguous HCV-1 subtypes.
  • To assess the reliability and efficiency of the GT Plus assay in a clinical setting.

Main Methods:

  • The GT Plus assay was tested on 198 HCV-1 specimens, including those with ambiguous results from the Abbott RealTime Genotype II (GT II) assay.
  • Sanger sequencing and comparison with LiPA 2.0 were used for validation.
  • Performance was evaluated based on successful subtyping rates and concordance with reference methods.

Main Results:

  • The GT Plus assay successfully assigned subtypes in 88.8% (142/160) of previously un-subtyped samples.
  • Overall subtyping concordance between GT Plus and reference methods (sequencing or LiPA 2.0) was high at 98.6% (140/142).
  • The combined use of GT II and GT Plus assays reduced ambiguous HCV-1 results, achieving 98.9% successful subtyping.

Conclusions:

  • The combined use of Abbott GT II and GT Plus assays provides a reliable and simple strategy to significantly reduce ambiguous HCV-1 results.
  • This approach enhances the overall efficiency and accuracy of HCV-1 subtyping in clinical diagnostics.

More Related Videos

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis06:30

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

Published on: February 5, 2014

23.0K
Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria10:27

Methods to Increase the Sensitivity of High Resolution Melting Single Nucleotide Polymorphism Genotyping in Malaria

Published on: November 10, 2015

12.1K

Related Concept Videos

Reliability and Validity01:29

Reliability and Validity

Reliability and validity are two important considerations that must be made with any type of data collection. Reliability refers to the ability to consistently produce a given result. In the context of psychological research, this would mean that any instruments or tools used to collect data do so in consistent, reproducible ways.
13.8K
What are Viruses?00:50

What are Viruses?

Overview
128.0K
Distribution Reliability and Automation01:25

Distribution Reliability and Automation

Distribution reliability in electrical power systems is critical for ensuring an uninterrupted power supply to consumers at minimal cost. According to IEEE Standard Terms, reliability is the probability that a device will function without failure over a specified time period or amount of usage. For electric power distribution, this translates to maintaining continuous power supply and addressing customer concerns over power outages. Several indices, as defined by IEEE Standard 1366-2012, are...
503
Effect of Hepatic Disease on Pharmacokinetics: Dose Adjustments Due to Hepatic Impairment01:08

Effect of Hepatic Disease on Pharmacokinetics: Dose Adjustments Due to Hepatic Impairment

Hepatic impairment, characterized by decreased liver function, does not uniformly mandate adjustments in drug dosage. Whether dosage modifications are necessary depends on various factors related to the drug's metabolism and elimination pathways. If a drug is primarily excreted via the kidneys and bypasses significant hepatic processing, if it undergoes minimal metabolic transformation in the liver, or if it is volatile and primarily expelled through the lungs, dose adjustments may not be...
269
Hepatic Portal System01:21

Hepatic Portal System

The hepatic portal system, a critical part of our circulatory framework, transports nutrient-laden, deoxygenated blood from the gastrointestinal tract and spleen to the liver. This ingenious system plays an indispensable role in maintaining our body's metabolic equilibrium.
At its core, the hepatic portal vein is the result of a confluence of the superior and inferior mesenteric veins along with the splenic vein. Each of these veins has a unique role. The superior mesenteric vein is...
5.7K
Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow01:26

Effect of Hepatic Disease on Pharmacokinetics: Drug Dosing and Hepatic Blood Flow

Chronic liver disease significantly impacts drug metabolism due to alterations in hepatic blood flow and enzyme accessibility. This disruption affects the body's pharmacokinetics—the movement and processing of drugs within the system. Key enzymes crucial for metabolizing medications become less accessible, changing how drugs are processed and utilized. Furthermore, liver disease influences the synthesis of plasma proteins, such as albumin and globulins, which play critical roles in drug...
224