Related Experiment Video
Updated: Jan 28, 2026
08:24
Bioinspired Soft Robot with Incorporated Microelectrodes
Published on: February 28, 2020
9.3K
Cell-Adhesive Bioinspired and Catechol-Based Multilayer Freestanding Membranes for Bone Tissue Engineering.
1CICECO-Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Biomimetics (Basel, Switzerland)
|March 8, 2019
Summary
Researchers developed mussel-inspired adhesive membranes using dopamine-modified hyaluronic acid and chitosan. These freestanding multilayer systems show enhanced adhesion and potential for bone tissue engineering applications.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Tissue Engineering
Background:
- Mussels exhibit remarkable underwater adhesion due to DOPA-rich proteins.
- Mimicking mussel adhesion is crucial for developing advanced biomaterials.
- Hyaluronic acid and chitosan are biocompatible polymers with potential in regenerative medicine.
Purpose of the Study:
- To synthesize a mussel-inspired conjugated polymer by modifying hyaluronic acid with dopamine.
- To fabricate freestanding multilayer membranes using chitosan and dopamine-modified hyaluronic acid (HA-DN) via layer-by-layer assembly.
- To evaluate the adhesive properties and potential of these membranes for bone tissue engineering.
Main Methods:
- Carbodiimide chemistry was employed to conjugate dopamine onto hyaluronic acid.
- Layer-by-layer (LbL) technique was utilized to create freestanding multilayer membranes from chitosan and HA-DN.
- Surface morphology, chemistry, and mechanical properties were characterized using various techniques.
- MC3T3-E1 cell culture was performed to assess biocompatibility and cell adhesion.
Main Results:
- Successful synthesis of dopamine-modified hyaluronic acid (HA-DN) confirmed by UV-vis and NMR spectroscopy.
- Freestanding multilayer membranes composed of chitosan and HA-DN were fabricated.
- Electrostatic interactions were identified as the primary driving force for film formation.
- Enhanced adhesive properties of the membranes were observed due to the incorporation of HA-DN.
- The membranes supported the culture of MC3T3-E1 cells, indicating good biocompatibility.
Conclusions:
- Mussel-inspired HA-DN is a promising material for enhancing the adhesive properties of biomaterial systems.
- The developed freestanding multilayer membranes demonstrate potential for bone tissue engineering applications.
- LbL assembly provides a versatile platform for creating hierarchical structures with tunable properties for regenerative medicine.
Related Concept Videos
01:30Bone Cells and Tissue
8.2K
Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
Osteoblasts and Osteocytes
The osteoblast is the bone cell responsible for forming new bone tissue. It is found in the growing portions of bone, including the...
8.2K
01:27Tissue Membranes
8.2K
A tissue membrane is a thin layer of cells that covers the outside of the body, the organs, internal passageways that lead to the exterior of the body, and the lining of the moveable joint cavities. There are two basic types of tissue membranes— connective tissue and epithelial membranes.
Connective Tissue Membranes
The connective tissue membrane is formed solely from connective tissue. These membranes encapsulate organs, such as the kidneys, and line our movable joints. A synovial...
Connective Tissue Membranes
The connective tissue membrane is formed solely from connective tissue. These membranes encapsulate organs, such as the kidneys, and line our movable joints. A synovial...
8.2K
01:17Hormones and Bone Tissue
3.8K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.8K
01:14Adhesion
43.9K
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...
Capillary action is a result of water’s adhesive tendencies. When a narrow...
43.9K
01:14Cell Adhesion in Plants
3.3K
Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose,...
3.3K
01:27Growth of Cartilage and Bone Tissue
4.2K
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
4.2K