Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

C4 Pathway and CAM01:27

C4 Pathway and CAM

49.1K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.1K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.4K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
19.4K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

885
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
885
Respiration Pathways01:26

Respiration Pathways

773
Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
773
Auditory Pathway01:15

Auditory Pathway

7.3K
Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...
7.3K
ER Retrieval Pathway01:45

ER Retrieval Pathway

4.8K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of dorsal striatal DNMT3b in alcohol seeking behavior after withdrawal.

Neuroscience letters·2026
Same author

A Deep-Red Emissive Cage-in-Rings Complex for Lysosome Imaging.

Angewandte Chemie (International ed. in English)·2026
Same author

Characterization of saponin biotransformation driven by intrinsic proteins in <i>Aralia elata</i> leaves.

Food chemistry: X·2026
Same author

Prevalence of psychotic symptoms and their clinical correlates in elderly major depressive disorder patients with subclinical hypothyroidism.

Neuropsychobiology·2026
Same author

Six Lanthanide Complexes: Crystal Structures, Spectroscopic Properties, and Pyrolysis Characteristics Analysis by Thermogravimetry-Differential Scanning Calorimetry/Fourier Transform Infrared/Mass Spectrometry.

Inorganic chemistry·2026
Same author

Markedly Strengthened Delayed Fluorescence and Processability in Crystalline Hybrids via Solvation-Enhanced Self-Dissolution-Recrystallization.

The journal of physical chemistry letters·2026

Related Experiment Video

Updated: Jan 28, 2026

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
05:16

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Published on: August 4, 2021

3.8K

Disordered p53-MALAT1 pathway is associated with recurrent miscarriage.

Yan Wang1, Hui-Ze Liu1, Yang Liu1

  • 1Department of Obstetrics, Affiliated Hospital of Weifang Medical University, Weifang, China.

The Kaohsiung Journal of Medical Sciences
|March 9, 2019
PubMed
Summary

Recurrent miscarriage (RM) involves unknown causes. This study shows p53 suppresses MALAT1 expression, impacting trophoblast cell functions crucial for pregnancy. MALAT1 partially reverses p53

Keywords:
MALAT1long noncoding RNAp53recurrent miscarriage

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.7K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K

Related Experiment Videos

Last Updated: Jan 28, 2026

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage
05:16

Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

Published on: August 4, 2021

3.8K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.7K
Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

10.0K

Area of Science:

  • Reproductive Biology
  • Molecular Genetics
  • Cell Biology

Background:

  • Recurrent miscarriage (RM) affects 1% of couples, with unknown causes in 50% of cases.
  • P53, a cell cycle regulator, influences long noncoding RNA (lncRNA) expression and is overexpressed in RM patients.
  • The role of p53 and lncRNAs in RM pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the relationship between p53 and specific lncRNAs in recurrent miscarriage.
  • To elucidate the mechanism by which p53 affects lncRNA expression and trophoblast function.

Main Methods:

  • Detected p53 and six lncRNA expression levels in villous tissue from RM patients and controls.
  • Utilized luciferase assays to confirm p53 binding to the MALAT1 promoter.
  • Performed functional studies using human trophoblast cells (HTR-8/SVneo) with p53 and MALAT1 overexpression.

Main Results:

  • NEAT1 and MALAT1 lncRNA levels were significantly reduced in RM patients.
  • MALAT1 levels showed a negative correlation with p53 protein levels.
  • p53 directly represses MALAT1 expression by binding to its promoter.
  • p53 overexpression decreased trophoblast cell proliferation, migration, and invasion, while increasing apoptosis.
  • MALAT1 overexpression partially rescued these functional deficits caused by p53 overexpression.

Conclusions:

  • P53-mediated repression of MALAT1 contributes to the pathogenesis of recurrent miscarriage.
  • Dysregulation of the p53-MALAT1 axis impacts trophoblast cell function, potentially leading to pregnancy loss.
  • Targeting the p53-MALAT1 pathway may offer therapeutic strategies for recurrent miscarriage.