Related Experiment Video
Updated: Jan 28, 2026
Growth of Gold Dendritic Nanoforests on Titanium Nitride-coated Silicon Substrates
Published on: June 3, 2019
The interactions of dendritic cells with osteoblasts on titanium surfaces: an in vitro investigation
Yang Yang1,2, Xuzhu Wang1, Richard J Miron3
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Objectives:
Osteoimmune interactions possess a critical part in the integration of materials and hosts. Dendritic cells (DCs) are the most common members of osteoimmune cells family. The titanium surfaces of dental implants tend to promote a mature dendritic cell phenotype with increased proinflammatory secretion. However, very little is known to the effects of this microenvironment on the behaviors of cells around implants, especially osteoblasts, and how the tissue integrations take place on such biomaterial surfaces. Therefore, the present study was to investigate the interactions of DCs with osteoblasts on titanium surfaces. DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions.
Materials And Methods:
DCs seeded on PT and SLA titanium surfaces were compared by assays for the proliferations, surface markers, and inflammatory genes expressions. Next, we harvested the dendritic cell-conditioned medium (CM) and investigated the effects of CM on MC3T3-E1.
Results:
The results showed an increase in CD86 and proinflammatory expressions of DCs seeded on PT and SLA surfaces, as well as a decrease in anti-inflammatory cytokines. The CM from titanium surfaces inhibited the osteoblast differentiation with reduced expression of osteogenic genes RUNX2, COL1, ALP, and OCN and decreased ALP activity as well as Alizarin red staining.
Conclusion:
These findings suggested that titanium surfaces switch DCs toward maturation phenotypes and thus inhibit the differentiation and mineralization of osteoblasts.
Clinical Relevance:
Knowing the significant impact of immune cells on osteogenesis behaviors, some efforts to decrease the immune reaction might be of clinical significance. Favorable immune environments can increase the dental implants survival rate in patients.
More Related Videos
11:14A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
Published on: January 10, 2017
07:05In Vitro Evaluation of The Effects Of Er,Cr:YSGG and Diode Lasers Used on Titanium Cylinder
Published on: June 6, 2025
Related Concept Videos
01:21Cell-surface Signaling
01:23Neurons: The Cell Body and the Dendrites
01:28Types of Receptors: Cell Surface Receptors
01:24Overview of Cell-Matrix Interactions
01:16Surface Tension and Surface Energy
Consider a beaker filled with liquid. The bulk molecules in the liquid experience equal attractive forces on all sides with the surrounding molecules. However, the surface molecules experience a net attractive force downward due to the bulk molecules. The surface of the liquid behaves like a stretched membrane,...
02:57Surface Tension, Capillary Action, and Viscosity
The various IMFs between identical molecules of a substance are examples of cohesive forces. The molecules within a liquid are surrounded by other molecules and are attracted equally in all directions by the cohesive forces within the liquid. However, the molecules on the surface of a liquid are attracted only by about one-half as many molecules. Because of the unbalanced molecular attractions on the surface molecules, liquids contract to form a shape that minimizes the number...