Boron reduces cell wall aluminum content in rice (Oryza sativa) roots by decreasing H₂O₂ accumulation

When boron (B) deficiency and aluminum (Al) toxicity co-exist in acidic soils, crop productivity is limited. In the current study, we found that 3 μM of B pretreatment significantly enhances rice root elongation under Al toxicity conditions. Pretreatment with B significantly decreases the deposition of Al in rice apoplasts, suppresses the synthesis of cell wall pectin, inhibits cell wall pectin methylesterase (PME) activity and its gene expression, and increases the expression of OsSTAR1 and OsSTAR2, which are responsible for reducing the Al content in the cell walls. In addition, B pretreatment significantly increases OsALS1 expression, thereby facilitating the transfer of Al from the cytoplasm to the vacuoles. However, B pretreatment had no effect on Al uptake and citric acid secretion. Pretreatment with B significantly increases the activity of ascorbate peroxidase (APX), peroxidase (POD), and catalase (CAT), thus increasing the elimination rate of H₂O₂ in rice roots. Co-treatment using B and H₂O₂ does not increase root growth under Al toxicity conditions; it also improves pectin synthesis, enhances PME activity, and increases Al deposition in root cell walls. However, the co-treatment of B and H₂O₂ scavenger 4-hydroxy-TEMPO has an opposite effect. The above results indicate that applying B fertilizers in acidic soil can help decrease the side effects of Al toxicity on rice growth.