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Updated: Jan 28, 2026

Spatiotemporal Subcellular Manipulation of the Microtubule Cytoskeleton in the Living Preimplantation Mouse Embryo using Photostatins
Published on: November 30, 2021
In vitro exposure to pyrethroid-based products disrupts development of mouse preimplantation embryos
Janka Babeľová1, Zuzana Šefčíková1, Štefan Čikoš1
1Institute of Animal Physiology, Centre of Biosciences, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovak Republic.
Abstract:
The objective of this study was to evaluate the potential toxicity of pyrethroids (deltamethrin, permethrin, fenvalerate, λ-cyhalothrin), commercial pyrethroid-based products DECIS EW 50 (deltamethrin mixture), TOP SPOT ON STRONGER (permethrin mixture), as well as related secondary ingredients on mouse preimplantation embryo development. Two-cell stage embryos were in vitro cultured with addition of the listed chemicals until blastocyst formation. All active pyrethroids negatively affected embryonic development at 1000 μM concentration. Decreased quality of obtained blastocysts in permethrin, fenvalerate and λ-cyhalothrin-treated embryos was revealed as well. Deltamethrin showed harmful impact on embryo development at 100 μM concentration. Lower concentrations of pyrethroids (1, 10 μM) had no effect on embryo development. The presence of DECIS EW 50 containing deltamethrin at 100 μM caused degeneration of all embryos. Similarly, TOP SPOT ON STRONGER containing 100 μM of permethrin impaired embryonic development and quality of obtained blastocysts. Evaluated secondary ingredients (butylhydroxyanisol, butylhydroxytoluen, butylparaben and cyclohexanone) at corresponding concentrations showed damaging impact on preimplantation embryo development as well. Our results indicate that the embryotoxic potential of active pyrethroids is relatively low, whereas pyrethroid-based products have relatively high potential to impair mouse preimplantation development. Embryotoxicity of commercial products is probably attributable to the presence of secondary ingredients.
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