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Related Experiment Video

Updated: Jan 28, 2026

Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
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Mast cell-mediated splanchnic cholestatic inflammation.

María-Ángeles Aller1, Vicente Martínez2, Ana Arias3

  • 1Department of Surgery, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain.

Clinics and Research in Hepatology and Gastroenterology
|March 12, 2019
PubMed
Summary
This summary is machine-generated.

Ketotifen, a mast cell stabilizer, improved liver function and reduced splanchnic inflammation in cholestatic rats. This suggests targeting mast cells may alleviate liver disease complications.

Keywords:
AscitesCholestasisMast cellsPortal hypertension

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Area of Science:

  • Hepatology and immunology research.
  • Gastroenterology and internal medicine.

Background:

  • Splanchnic mast cells are implicated in chronic and acute-on-chronic liver diseases.
  • Cholestasis leads to systemic and splanchnic impairments, including ascites and portal hypertension.
  • Inflammatory mediators and vascular endothelial growth factor (VEGF) are altered in cholestatic conditions.

Purpose of the Study:

  • To investigate the effect of Ketotifen, a mast cell stabilizer, on splanchnic organs in a rat model of cholestasis.
  • To evaluate the impact of Ketotifen on mast cell populations and inflammatory markers in cholestatic rats.

Main Methods:

  • A microsurgical cholestasis model was established in rats.
  • Rats were divided into sham-operated, untreated cholestatic, and Ketotifen-treated groups (early and late administration).
  • Mast cell counts, inflammatory mediators (TNF-α, IL-1β, MCP-1, IL-4, IL-10), VEGF, and TGF-β1 levels were measured.

Main Results:

  • Cholestatic rats exhibited ascites, portal hypertension, fibrosis, and increased splanchnic TNF-α, IL-1β, and MCP-1.
  • Ketotifen treatment reduced hepatic insufficiency, splanchnic inflammatory mediators, VEGF, and TGF-β1 levels.
  • Ketotifen decreased connective tissue mast cells in the mesenteric lymphatic complex and increased hepatic mucosal mast cells.

Conclusions:

  • Ketotifen administration improves liver function and reduces ascites in cholestatic rats.
  • Reducing splanchnic connective tissue mast cells with Ketotifen ameliorates the inflammatory response and associated complications.
  • Targeting mast cells offers a potential therapeutic strategy for liver disease complications.