Related Experiment Video
Updated: Jan 28, 2026

Studying Orthodontic Tooth Movement in Mice
Published on: August 2, 2024
Correlation between pain and hyalinization during tooth movement induced by different types of force
Objectives:
To evaluate the correlation between pain and tissue reactions during induced tooth movement (ITM).
Materials And Methods:
Forty-two male Wistar rats (Rattus norvegicus; ∼90 days of age, 300 g) were used. The animals were divided into seven groups of six rats each: one control group and six experimental groups subjected to ITM by continuous force (CF) or interrupted continuous force (ICF) for 1, 3, and 5 days. Hyalinization of the periodontal ligament (PL) and occurrence of pain were observed. Animal behavior (walking, climbing, immobile posture, resting/sleeping, and directed face grooming) and the presence of chemical mediators associated with nociception, cyclooxygenase-2 (COX-2), and interleukin-1 beta (IL-1β) in the PL were analyzed.
Results:
There was a moderate positive correlation between hyalinization and the presence of COX-2 (rs = 0.404; P < .05) and IL-1β (rs = 0.429; P < .05). There was a moderate negative correlation between hyalinization and exploratory behaviors (walking, r = -0.586, P < .01; climbing, r = -0.573, P < .01), and a moderate positive correlation between hyalinization and resting/sleeping (r = 0.467; P < .01).
Conclusions:
The results suggest a correlation between pain and undesirable tissue reactions in ITM.
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