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Adhesion of Methicillin-Resistant Staphylococcus aureus and Candida albicans to Parylene-C-Coated Polymethyl

Ingrid M Green, Ioanna Margoni, Sean P Nair

    The International Journal of Prosthodontics
    |March 12, 2019
    PubMed
    Abstract

    Insights

    Coating polymethyl methacrylate (PMMA) with Parylene-C did not prevent Staphylococcus aureus or Candida albicans biofilm formation. This study found no significant difference in microbial counts on coated versus uncoated PMMA discs.

    Area of Science:

    • Biomaterials Science
    • Microbiology
    • Dental Materials

    Background:

    • Polymethyl methacrylate (PMMA) is widely used in dental prosthetics.
    • Biofilm formation on PMMA can lead to device failure and infections.
    • Controlling microbial adhesion on PMMA surfaces is crucial for clinical success.

    Purpose of the Study:

    • To evaluate the efficacy of Parylene-C coating in preventing biofilm formation.
    • To assess the impact of Parylene-C on Staphylococcus aureus and Candida albicans adhesion to PMMA.

    Main Methods:

    • Single and dual-species biofilms of MRSA and Candida albicans were cultured on coated and uncoated PMMA discs for 48 hours in artificial saliva.
    • Viable biofilm colony-forming units (CFUs) were quantified to assess microbial load.

    Main Results:

    • No statistically significant difference was observed in the viable counts of MRSA or Candida albicans between Parylene-C coated and uncoated PMMA discs.
    • The presence of Parylene-C coating did not inhibit the formation of single or dual-species biofilms.

    Conclusions:

    • Parylene-C coating does not appear to be an effective strategy for preventing Staphylococcus aureus and Candida albicans biofilm formation on PMMA.
    • Further research may be needed to explore alternative surface modifications for PMMA to combat biofilm.

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