Related Experiment Video
Updated: Jan 28, 2026

Posterior Semicircular Canal Approach for Inner Ear Gene Delivery in Neonatal Mouse
Published on: March 2, 2018
The effects of pressure in vitro on three methods of root canal obturation
Pooria Safai1, Babak Farzaneh1,2, Reza Fekrazad3,4
1Department of Endodontics, Faculty of Dentistry, AJA University of Medical Sciences, Tehran, Iran.
Introduction:
The seal of root canal fillings depends on the anatomy of the root canal system and on the properties of filling materials and their bond to the walls of the root canal. Alterations in pressure may influence these properties. The current study investigated and compared microleakage in a tapered single-cone method versus lateral and vertical condensation after diving simulation.
Methods:
One hundred and thirty five extracted single-rooted teeth were used. Following instrumentation and irrigation to #30.06 Mtwo rotary system, obturations were performed in three groups of 45 teeth: Group 1, tapered single-cone with Endoseal MTA sealer; Group 2, lateral condensation with AH26 sealer; Group 3, vertical condensation with AH26 sealer. Then all specimens were divided into three groups and incubated at ambient room pressure (101.3 kPa), 203 kPa or 304 kPa for 120 minutes respectively 20 times over one month to simulate diving conditions. Microleakage quantitative analysis was recorded by using a 2% Methylene blue dye for 24 hours.
Results:
The amounts of microleakage increased with increasing pressure in all obturation groups; however, the differences were not statistically significant (P > 0.05). At all three pressures, the least microleakage was recorded in Group 3, vertical condensation. Although the differences between vertical condensation, lateral condensation, and tapered single-cone methods were statistically significant (P < 0.001), the vertical condensation and lateral condensation groups did not differ from each other (P > 0.05).
Conclusions:
Vertical condensation may be the best technique, based on sealing ability, for people who frequently experience pressure alterations.
More Related Videos
08:21Effects of Blast-induced Neurotrauma on Pressurized Rodent Middle Cerebral Arteries
Published on: April 1, 2019
09:17Dorsal Root Ganglia Neurons and Differentiated Adipose-derived Stem Cells: An In Vitro Co-culture Model to Study Peripheral Nerve Regeneration
Published on: February 26, 2015
Related Concept Videos
Root-Locus Method
This system can be represented by a block...
Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
Primary and Secondary Growth in Roots and Shoots
Vapor Pressure
Assessment of blood pressure in brachial artery(one-step method)
Prepare for the Procedure:
Assessment of blood pressure in brachial artery(two-step method)