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Updated: Jan 27, 2026
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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
Published on: August 16, 2016
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Detection of Single Proteins with a General Nanopore Sensor
Qiao Li1, Yi-Lun Ying1, Shao-Chuang Liu1
1Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering , East China University of Science and Technology , Shanghai , 200237 , P. R. China.
ACS Sensors
|March 13, 2019
Summary
We developed a new method using protein cross-linking to slow down single proteins in nanopore sensors. This allows for more accurate detection and study of protein dynamics at the single-molecule level.
Area of Science:
- Biophysics
- Nanotechnology
- Biochemistry
Background:
- Single-molecule protein detection using solid-state nanopores is challenging due to rapid protein translocation speeds exceeding instrument bandwidth.
- Existing nanopore sensing techniques struggle with the high velocity of proteins, limiting sensitivity and resolution.
Purpose of the Study:
- To develop a general and robust nanopore sensing platform for single-molecule protein detection by decelerating translocation speed.
- To investigate the anisotropic translocation dynamics of proteins within nanopore confinement.
Main Methods:
- Utilized a common protein cross-linking interaction (EDC/NHS coupling) to slow down protein translocation through solid-state nanopores.
- Analyzed distinguishable current signatures generated during protein translocation events.
Main Results:
- Achieved a 10-fold decrease in protein translocation speed through the nanopore.
- Observed and characterized anisotropic translocation behaviors including horizontal, vertical, and flipping transits.
- Demonstrated a robust platform for single-molecule protein detection.
Conclusions:
- The EDC/NHS coupling strategy provides a general platform for rapid single-molecule protein detection.
- This approach enables the exploration of fundamental protein dynamics at the single-molecule level.
- Decelerating translocation velocity enhances the capability of nanopore instruments for protein analysis.
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