Quantifying protein dynamics and stability in a living organism
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Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
Ruopei Feng1, Martin Gruebele2,3,4, Caitlin M Davis5,6
1Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Abstract:
As an integral part of modern cell biology, fluorescence microscopy enables quantification of the stability and dynamics of fluorescence-labeled biomolecules inside cultured cells. However, obtaining time-resolved data from individual cells within a live vertebrate organism remains challenging. Here we demonstrate a customized pipeline that integrates meganuclease-mediated mosaic transformation with fluorescence-detected temperature-jump microscopy to probe dynamics and stability of endogenously expressed proteins in different tissues of living multicellular organisms.
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