Related Experiment Video
Updated: Jan 27, 2026

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture
Published on: April 10, 2020
Acylated Anthocyanins in Red Onions
A B Moore1, F J Francis1, M E Jason1
1Department of Food Science and Nutrition, University of Massachusetts, Amherst, Massachusetts, 01003 and Department of Chemistry, Amherst College, Amherst, Massachusetts 01002.
Abstract:
Chromatographic fractions of the onion pigment profile were examined for acyaltion by alkaline hydrolysis, visible/UV spectral, IR spectral, GLC, and NMR techniques. Results indicative of acylation were shown by alkaline hydrolysis and IR spectral analyses. The suspected acyl portion was shown not to be a cinnamic acid by visible/UV spectral analysis. NMR and GLC methods failed to detect acylation in the onion pigment system. Small amounts of assay material, low sensitivity for detection of some of the possible acyl acids, removal of the acyl by complex formation, loss of the acyl due to high volatility and low acid transfer in partitioning systems are some of the factors identified as interfering with acyl detection.
More Related Videos
Related Concept Videos
Amines to Amides: Acylation of Amines
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
Red Algae
Nucleophilic Acyl Substitution of Carboxylic Acid Derivatives
Electrophilic Aromatic Substitution: Friedel–Crafts Acylation of Benzene
tRNA Activation
Photoreceptors and Plant Responses to Light

