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Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
Published on: April 13, 2019
Changes in the Natural Microflora During Incubation of Fresh and Processed Human Milks 1
N Shewakramani1, M O Hanna1, E S Alford1
1Animal Science Department, Texas A&M University, College Station, Texas 77843 and Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030.
Abstract:
Seven pooled human milks, each from at least 6 different breast-feeding mothers (4 weeks post-partum) were studied for microbial population and flora changes during storage at 37°C. Coagulase-negative Staphylococcus spp. constituted a considerable part (39-100%) of the microflora in 6 of 7 pools of fresh milk, with coryneform bacteria, Lactobacillus , Micrococcus and Streptococcus spp. isolated less frequently. Storage of raw milk for 8 h caused a shift in flora favoring the Lactobacillus spp. After 24 h, coagulase-negative Staphylococcus were dominant in 4 of 7 pools, coryneform bacteria in 2 of 7, and Lactobacillus and Micrococcus spp. each in 1 of 7 pools. Low levels of bacteria were detected in 2 of 7 pasteurized pools, consisting of Staphylococcus spp. or yeasts. Freezing of the milk before storage at 37°C had little effect on the aerobic plate counts.
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