Expression and Functional Analysis of Two Odorant-Binding Proteins from Bradysia odoriphaga (Diptera: Sciaridae)
Bowen Tang1, Shulei Tai1, Wu Dai1
1Key Laboratory of Plant Protection Resources and Pest Integrated Management of the Ministry of Education, College of Plant Protection , Northwest A&F University , Yangling , Shaanxi 712100 , P. R. China.
Journal of Agricultural and Food Chemistry
|March 15, 2019
Summary
Two odorant-binding proteins (OBPs) from the pest Bradysia odoriphaga are crucial for detecting host plant volatiles and female sex pheromones, impacting agricultural pest control strategies.
Area of Science:
- Molecular Entomology
- Insect Chemical Ecology
- Agricultural Entomology
Background:
- Bradysia odoriphaga is a significant agricultural pest affecting Chinese chives.
- Odorant-binding proteins (OBPs) are essential for insect olfaction, mediating the detection of environmental chemical cues.
- Understanding OBPs in pest species is critical for developing targeted control strategies.
Purpose of the Study:
- To clone and characterize two OBPs (BodoOBP1 and BodoOBP2) from Bradysia odoriphaga.
- To investigate the role of these OBPs in the detection of host plant volatiles and sex pheromones.
- To explore the potential of these OBPs as targets for pest management.
Main Methods:
- Gene cloning and sequence analysis of BodoOBP1 and BodoOBP2.
- Fluorescence competitive binding assays to determine ligand affinity.
- Quantitative real-time PCR for tissue and developmental expression profiling.
- RNA interference (RNAi) assays to assess functional roles in behavior.
Main Results:
- BodoOBP1 and BodoOBP2 exhibit high sequence similarity and moderate binding affinity to dipropyl trisulfide, a plant volatile.
- Both OBPs are antennae-specific, with higher expression in males and adult stages, and show differential expression in relation to mating.
- RNAi-mediated knockdown of BodoOBPs significantly impaired male detection of females and attraction to dipropyl trisulfide.
Conclusions:
- BodoOBP1 and BodoOBP2 play vital roles in the host-seeking behavior of Bradysia odoriphaga.
- These OBPs are involved in detecting both plant volatiles and sex pheromones, suggesting a dual function in olfaction.
- The characterized OBPs represent potential targets for novel pest control strategies against Bradysia odoriphaga.
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