Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Duplication and Divergence02:37

Gene Duplication and Divergence

7.9K
The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
7.9K
Centrosome Duplication02:25

Centrosome Duplication

5.0K
The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
5.0K
Fruit Development, Structure, and Function01:58

Fruit Development, Structure, and Function

25.1K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.1K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

7.4K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
7.4K
Gene Families01:57

Gene Families

9.9K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
9.9K
Requirements for Human Life01:26

Requirements for Human Life

13.4K
The Earth and its atmosphere have provided humans with air, water, and food, but these are not the only requirements for survival. Humans also require a specific range of temperature and pressure that the Earth and its atmosphere provides.
Oxygen
Atmospheric air is only about 20 percent oxygen, but that oxygen is a key component of the chemical reactions that keep the body alive, including the reactions that produce ATP. Brain cells are susceptible to a lack of oxygen because they require a...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human brain pericytes protect the blood-brain barrier from triple-negative breast cancer cells while promoting tumor aggressiveness.

Journal of cell communication and signaling·2026
Same author

<i>H19</i>/miR-675 Axis Promotes Cancer Metastasis by Orchestrating EMT and MET Processes.

Cells·2026
Same author

Spatial self-organization of cancer stem cell niches revealed by live single-cell imaging.

Stem cell research & therapy·2025
Same author

Ezh2 Loss-of-Function Alters Zebrafish Cerebellum Development.

International journal of molecular sciences·2025
Same author

Retraction of "Cell Adhesion Properties on Chemically Micropatterned Boron-Doped Diamond Surfaces".

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

EZH2 specifically regulates ISL1 during embryonic urinary tract formation.

Scientific reports·2024

Related Experiment Video

Updated: Jan 27, 2026

Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

78.8K

Ezh1 arises from Ezh2 gene duplication but its function is not required for zebrafish development.

Pamela Völkel1,2,3, Aurélie Bary1,2,4, Ludivine Raby1,2

  • 1Inserm U908, Cell Plasticity & Cancer, Lille, France.

Scientific Reports
|March 15, 2019
PubMed
Summary

Polycomb Repressive Complex 2 (PRC2) trimethylation of H3K27 is crucial for development. In zebrafish, Ezh1, maternally deposited, compensates for Ezh2 loss, delaying lethality in mutant larvae.

More Related Videos

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
10:39

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Published on: April 22, 2017

11.8K
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
07:19

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Published on: September 28, 2012

11.9K

Related Experiment Videos

Last Updated: Jan 27, 2026

Microinjection of Zebrafish Embryos to Analyze Gene Function
07:18

Microinjection of Zebrafish Embryos to Analyze Gene Function

Published on: March 9, 2009

78.8K
Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
10:39

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Published on: April 22, 2017

11.8K
Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
07:19

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Published on: September 28, 2012

11.9K

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Polycomb Repressive Complex 2 (PRC2) mediates H3K27 trimethylation, a key epigenetic mark for gene repression.
  • In mammals, Ezh1 and Ezh2 are PRC2 catalytic subunits, while Drosophila has a single E(z) protein.
  • Ezh1 is hypothesized to have evolved from an Ezh2 gene duplication in early vertebrates.

Purpose of the Study:

  • To investigate the evolutionary origin and function of Ezh1 in zebrafish development.
  • To characterize the expression pattern of ezh1 during zebrafish embryogenesis.
  • To determine the role of Ezh1 in the absence of functional Ezh2.

Main Methods:

  • Database searches for evolutionary analysis.
  • Zebrafish in situ hybridization and RT-PCR for expression studies.
  • TALEN technology for generating Ezh1-deficient zebrafish mutants.

Main Results:

  • Ezh1 mRNA is maternally deposited and ubiquitously expressed early in zebrafish development, later becoming restricted to the anterior.
  • Ezh1-deficient zebrafish are viable and fertile.
  • Loss of Ezh1 function leads to earlier lethality in ezh2 mutant larvae, indicating a compensatory role.

Conclusions:

  • Ezh1 plays a significant role in zebrafish development, particularly when zygotic Ezh2 function is absent.
  • Maternally provided Ezh1 transcripts contribute to the viability of ezh2-deficient zebrafish larvae.
  • Ezh1's function highlights its importance in compensating for Ezh2 during early development.