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First report of molecular characterization and phylogenetic analysis of Sarcocystis tenella from India

Vikrant Sudan1, Rahul Kumar2, Daya Shanker2

  • 1College of Veterinary Sciences &Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India. viks.sudan@gmail.com.

Parasitology Research
|March 15, 2019
PubMed

Insights

This study presents the first molecular characterization of Sarcocystis tenella in India using ribosomal 18S rRNA and mitochondrial cox1 genes. Findings reveal genetic diversity within Indian isolates and their relationship to global Sarcocystis tenella populations.

Area of Science:

  • Veterinary Parasitology
  • Molecular Biology
  • Ovine Pathology

Background:

  • Sarcocystis tenella is a widespread coccidian parasite affecting sheep globally, with high prevalence rates.
  • Existing Indian reports on S. tenella rely solely on morphology, lacking molecular data.
  • This study addresses the gap in molecular characterization of S. tenella in the Indian subcontinent.

Purpose of the Study:

  • To perform the first molecular characterization of Sarcocystis tenella from India.
  • To analyze genetic diversity using 18S rRNA and mitochondrial cox1 genes.
  • To conduct phylogenetic analysis and compare Indian isolates with global strains.

Main Methods:

  • Molecular characterization of five Indian S. tenella isolates using 18S rRNA and mitochondrial cox1 genes.
  • Sequencing and submission of genetic data to NCBI.
  • Phylogenetic analysis alongside standard histopathology of sarcocysts.

Main Results:

  • Identification of two haplotypes for both 18S rRNA and cox1 genes within Indian isolates.
  • High nucleotide homology (99.8-100.0% for 18S rRNA, 99.7-100.0% for cox1) among Indian isolates.
  • Significant nucleotide homology (93.3-99.9% for 18S rRNA, 95.2-99.8% for cox1) with global S. tenella sequences.
  • Phylogenetic analysis placed Indian S. tenella with a monophyletic cluster, sister to S. gracilis and S. alces.

Conclusions:

  • This study provides the foundational molecular data for Sarcocystis tenella in India.
  • The genetic analysis reveals diversity within Indian isolates and confirms their relationship to global populations.
  • The findings contribute to a better understanding of Sarcocystis epidemiology and evolution in sheep.
Keywords:
18S rRNAMolecular characterizationPhylogenetic analysisSarcocystis tenellacox1

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