Related Experiment Video
Updated: Sep 22, 2025
07:27
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
Published on: April 18, 2016
10.6K
An endpoint visualization loop-mediated isothermal amplification (LAMP) for detecting bubaline theileriosis
Sanjeev Kumar1, Sanjhi Paliwal2, Vikrant Sudan1
1Department of Parasitology, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
Beni-Suef University Journal of Basic and Applied Sciences
|May 18, 2022
Summary
Loop-mediated isothermal amplification (LAMP) is a highly sensitive and rapid diagnostic tool for tropical theileriosis in buffaloes. This novel method outperforms traditional PCR and microscopy for detecting Theileria annulata infection.
Area of Science:
- Veterinary Parasitology
- Molecular Diagnostics
- Apicomplexan Diseases
Background:
- Tropical theileriosis, caused by Theileria annulata, significantly impacts buffalo health and productivity in India.
- Accurate diagnosis is crucial for effective control programs during outbreaks.
- Limited diagnostic assays are available specifically for bubaline theileriosis compared to cattle.
Purpose of the Study:
- To evaluate the efficacy of Loop-mediated isothermal amplification (LAMP) for diagnosing tropical theileriosis in buffaloes.
- To compare the performance of LAMP with Polymerase Chain Reaction (PCR) and blood microscopical examination.
- To establish LAMP as a reliable diagnostic method for bubaline theileriosis.
Main Methods:
- Application of Loop-mediated isothermal amplification (LAMP) assay for deoxyribonucleic acid (DNA) detection.
- Comparison of LAMP results with Polymerase Chain Reaction (PCR) and blood microscopy.
- Assessment of diagnostic parameters including sensitivity, specificity, and kappa values.
Main Results:
- LAMP detected Theileria annulata infection in 27 out of 100 buffaloes.
- Blood microscopy identified 16 positive cases, while PCR detected 24 positive cases.
- No cross-reactivity was observed with DNA from other haemoprotozoans.
Conclusions:
- LAMP demonstrated significantly higher sensitivity, specificity, and kappa values compared to PCR and blood microscopy.
- This study reports the first-ever application of LAMP for detecting theileriosis in buffaloes globally.
- LAMP offers a rapid, sensitive, and instrument-minimal alternative for diagnosing tropical theileriosis in buffaloes.
