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Advances In Research On Genome Editing Crispr-Cas9 Technology
Syed Zawar Shah1, Anum Rehman1, Hira Nasir1
1Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan.
Journal of Ayub Medical College, Abbottabad : JAMC
|March 15, 2019
Summary
CRISPR gene editing technology, utilizing CRISPR/Cas9 and CRISPR/Cpf1 systems, offers precise DNA cleavage for genomic manipulation. Safe and ethical regulations are crucial for its research and clinical applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has evolved from a bacterial immune system into a revolutionary genome engineering technology.
- This technology is rapidly advancing in its mechanisms, functions, and applications across various fields.
Purpose of the Study:
- To elucidate the different aspects of CRISPR technology in light of current advancements.
- To review the mechanism, function, and applicability of CRISPR systems.
Main Methods:
- A systematic literature review was conducted.
- Information was gathered from all available online resources.
Main Results:
- CRISPR/Cas9 uses a Cas9 nuclease and CRISPR RNA (crRNA) for precise DNA cleavage.
- CRISPR/Cpf1 offers a simpler alternative with a smaller endonuclease (Cpf1).
- CRISPR technology enables genomic manipulation due to its simplicity, accuracy, and speed, but faces challenges like off-target effects.
Conclusions:
- CRISPR/Cas9 systems are poised to revolutionize the study and treatment of immunologic and allergic diseases.
- Safe and ethical regulations are necessary for the responsible implementation of CRISPR technology in research and clinical settings.
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