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Updated: Jan 27, 2026
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Gamete Collection and In Vitro Fertilization of Astyanax mexicanus
Published on: May 25, 2019
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[Cryoconservation of gametes: how to perform?]
Jeanne Perrin1, Patricia Fauque2, Marine Paci3
1Laboratoire de biologie de la reproduction- CECOS, AP-HM, La Conception, Marseille, France. Aix Marseille Univ, CNRS, IRD, Avignon Univ, IMBE UMR 7263, Marseille, France.
La Revue Du Praticien
|March 15, 2019
Summary
Patients can preserve fertility by cryopreserving sperm or oocytes before gonadotoxic treatments. Fertility preservation services coordinate care, offering options like sperm freezing and oocyte vitrification for future assisted reproductive treatments (ART).
Area of Science:
- Reproductive Medicine
- Oncology
- Bioethics
Background:
- Patients undergoing gonadotoxic treatments face fertility risks.
- French bioethical law mandates informing patients about fertility risks and preservation options.
- Regional fertility preservation platforms coordinate care between oncology and CECOS (Centers for Gamete and Embryo Preservation).
Purpose of the Study:
- To outline the procedures for gamete cryopreservation.
- To inform patients about fertility preservation options.
- To ensure ethical and legal compliance in fertility preservation.
Main Methods:
- Sperm cryopreservation involves a slow, controlled-temperature protocol.
- Oocyte cryopreservation typically uses vitrification after hormonal stimulation and oocyte retrieval.
- Cryopreserved gametes are stored in liquid nitrogen until needed for assisted reproductive treatment (ART).
Main Results:
- Established protocols exist for both male (sperm) and female (oocyte) gamete cryopreservation.
- Cryopreserved gametes are stored long-term in liquid nitrogen.
- Annual patient re-consent is obtained for continued cryopreservation.
Conclusions:
- Gamete cryopreservation offers a viable fertility preservation strategy for patients at risk of gonadotoxicity.
- Cryopreserved gametes can be used in ART during the patient's lifetime with their consent.
- The duration of cryopreservation does not alter gamete viability for ART.
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