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Updated: Jan 27, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Identification of a novel biomarker-CCL5 using antibody microarray for colorectal cancer
Mingxia Chen1, Xiaoqing Yang2, Min Yang2
1Department of Pathology, Qian-FO-Shan Hospital Affiliated Shandong University, 16766 Jingshi Road, Jinan 250014, China; Department of Pathology, Yatai Yaitaishan Hospital, 91 Jiefang Road, Yatai 264001, China.
Purpose:
To screen novel candidate biomarkers in primary colorectal cancer (CRC), and indentify their clinical valuation in progress of colorectal cancer.
Methods:
By using antibody microarray, 274 target proteins in tissue samples from primary colorectal cancer patients were detected. Among differently expressed proteins in CRC tissues, As promising candidate biomarker, RANTES/CCL5 was validated by enzyme-linked immunosorbentassay and immunohistochemistry (IHC), and the clinical significance of CCL5 was analyzed.
Results:
Totally, 25 differentially expressed proteins were indentified between colorectal cancers and matched normal mucosa. CCL5 expression was significantly associated with adverse pathological progress, apt to lymph node metastasis and higher T stage.
Conclusions:
CCL5 may contribute to promoting tumor growth, and CCL5 is a promising target that may help in understanding the pathogenesis of CRC.
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