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Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
Published on: July 27, 2016
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Modulation of protein oligomerization: An overview.
1Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India.
Progress in Biophysics and Molecular Biology
|March 16, 2019
Summary
Protein oligomerization, crucial for evolution, can be modulated. Understanding these protein interactions offers potential for future drug design targeting diseases linked to protein aggregation.
Area of Science:
- Biochemistry
- Molecular Biology
- Protein Science
Background:
- Many cellular proteins, in both prokaryotic and eukaryotic systems, exhibit oligomeric properties.
- Protein oligomerization is a significant phenomenon in protein evolution.
- Dysregulation of protein oligomerization is implicated in various diseases.
Purpose of the Study:
- To provide an overview of the modulation of protein oligomerization.
- To highlight the potential of modulating protein oligomerization for future drug design.
- To discuss methods for characterizing and regulating protein oligomerization.
Main Methods:
- Review of common and infrequent mechanisms of protein oligomerization, including domain swapping and ligand-induced dimerization.
- Discussion of regulatory mechanisms such as point mutations, post-translational modifications, and ligand binding.
- Overview of biophysical (microscopy, spectroscopy) and biochemical methods for characterizing oligomeric proteins.
Main Results:
- Ligand-induced oligomerization is a predominant and useful method for regulating protein oligomerization.
- Protein oligomerization can be functionally modulated both in vitro and in vivo using artificial and natural modulators.
- Characterization of oligomeric proteins can be achieved through various biophysical and biochemical techniques.
Conclusions:
- Modulation of protein oligomerization, through various natural and artificial means, is key to understanding protein function.
- Effective drug design strategies can be developed by targeting protein oligomerization pathways.
- Further research into protein oligomerization modulators holds promise for treating diseases associated with protein aggregation.
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