Related Experiment Video
Updated: Jan 27, 2026
04:10
The Use of Carboxyfluorescein Diacetate Succinimidyl Ester CFSE to Monitor Lymphocyte Proliferation
Published on: October 12, 2010
93.7K
Evaluation of a new lymphocyte proliferation assay based on cyclic voltammetry; an alternative method
Mohammad Nikbakht1, Babak Pakbin2, Gholamreza Nikbakht Brujeni3
1Department of Electronic Engineering, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.
Scientific Reports
|March 16, 2019
Summary
Cyclic voltammetry offers a simpler, faster method for monitoring lymphocyte proliferation and cell-mediated immunity compared to traditional assays. This electrochemical technique provides accurate results using fresh blood, reducing complexity and equipment needs.
Area of Science:
- Immunology and Electrochemistry
- Development of novel in vitro diagnostic tools
Background:
- Lymphocyte proliferation assays are crucial for assessing cell-mediated immunity.
- Existing in vitro methods are complex, requiring specialized equipment and reagents.
Purpose of the Study:
- To develop a novel, label-free electrochemical assay for cell proliferation and viability.
- To establish cyclic voltammetry as a simplified alternative to conventional lymphocyte proliferation assays.
Main Methods:
- Fabrication of a novel procedure based on the voltammetric behavior of proliferating cells.
- Monitoring cell proliferation in cell cultures and whole blood using cyclic voltammetry.
- Comparison of cyclic voltammetry with the colorimetric MTT assay.
Main Results:
- Cyclic voltammetry effectively monitored cell proliferation electrochemically.
- The electrochemical assay showed the best correlation with cell count data compared to MTT assay.
- Assay duration was significantly reduced to 4 hours, with rapid results.
Conclusions:
- Cyclic voltammetry provides an efficient, simple platform for electrochemical studies of lymphocyte proliferation.
- The method allows for the use of fresh, unprocessed blood, enhancing accuracy.
- This technique offers a promising alternative for label-free in vitro assessment of cell proliferation and viability.
More Related Videos
Related Concept Videos
01:10Voltammetric Techniques: Cyclic Voltammetry
1.6K
Cyclic voltammetry (CV) is an electrochemical technique used to investigate the redox properties of a chemical species. It involves measuring the current response of an electrochemical cell as a function of the applied potential. The setup for cyclic voltammetry typically consists of a working electrode, a reference electrode, and a counter electrode—all immersed in an electrolyte solution. The working electrode is where the redox reaction of interest occurs, while the reference electrode...
1.6K
01:13Voltammetry: Stripping Methods
874
Anodic Stripping Voltammetry (ASV), Cathodic Stripping Voltammetry (CSV), and Adsorptive Stripping Voltammetry (AdSV) are electrochemical techniques used to determine trace amounts of analytes in solution. These methods involve applying a potential to an electrode and measuring the resulting current.
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
Anodic Stripping Voltammetry (ASV)
ASV is used to determine metals and metalloids at trace levels. It involves two steps: deposition and stripping. First, a negative potential is applied to the...
874
01:24Area Computation by the Alternative Coordinate Method
601
The alternative coordinate method, also known as the Shoelace Formula, is a technique for determining the area of a traverse using Cartesian coordinates. This method relies on the sequential arrangement of x and y coordinates for each point of the shape, ensuring accuracy and ease of application.In this approach, each corner's x and y coordinates are listed as fractions, with the x-coordinate as the numerator and the y-coordinate as the denominator. These coordinates are arranged sequentially...
601
01:20Voltammetry: Overview
2.9K
Voltammetry is an electroanalytical technique in which the current flowing through an electrochemical cell is measured as a function of applied potential, typically under conditions of concentration polarization. The technique provides valuable information about redox-active species, and the current response is plotted as a voltammogram.
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
A voltammetric cell uses three electrodes: a working electrode, a reference electrode, and an auxiliary electrode. The redox reactions occur in the working...
2.9K
01:17In Vitro Drug Dissolution: Alternative Methods
219
Alternative drug dissolution methods include the rotating bottle, intrinsic dissolution test, peristalsis, and the Franz diffusion cell method. The rotating bottle method involves meticulously rotating tightly capped controlled-release beads in a temperature-controlled bath. Periodic decanting of samples allows for residue assay, followed by refilling with fresh medium and testing at various pH levels to emulate the gastrointestinal tract conditions.In contrast, the intrinsic dissolution test...
219
01:21Voltammetry: Factors Affecting Measurements
583
A current produced due to the redox reactions of the analyte at the working and auxiliary electrodes is called a faradaic current. The reaction can be divided into two types. The current generated due to the reduction of the analyte is called cathodic current, and it carries a positive charge. In contrast, the current produced by analyte oxidation is known as an anodic current, and it has a negative charge. The applied potential at the working electrode determines the faradaic current flow, and...
583