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Shotgun Proteomics of Human Dentin with Different Prefractionation Methods.
Matthias Widbiller1, Helmut Schweikl2, Astrid Bruckmann3
1Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany. matthias.widbiller@ukr.de.
Scientific Reports
|March 16, 2019
Summary
Human dentin contains numerous bioactive proteins released during demineralization. This study identified 813 proteins, offering insights into their functions and potential for dental pulp regeneration.
Area of Science:
- Biochemistry
- Proteomics
- Dental Research
Background:
- Human dentin is a mineralized tissue providing structural integrity to teeth.
- Dentin harbors bioactive proteins released upon demineralization, influencing pulp responses.
- Understanding dentin's protein composition is crucial for regenerative dentistry.
Purpose of the Study:
- To comprehensively analyze the human dentin proteome.
- To identify bioactive proteins within dentin and their functions.
- To explore the potential of dentin proteins in dental pulp regeneration.
Main Methods:
- Shotgun proteomics combined with three protein fractionation techniques: EDTA extraction, SDS-PAGE, OFFGEL IEF, and SCX.
- Liquid chromatography tandem mass spectrometry (LC-MS/MS) for protein identification.
- PANTHER system for protein categorization and overrepresentation analysis.
Main Results:
- Identification of 813 human proteins in dentin with high confidence.
- Isoelectric focusing (IEF) emerged as the most effective prefractionation method.
- Overrepresentation analysis revealed 31 protein classes and subclasses, highlighting key functional groups.
Conclusions:
- This study provides the first comprehensive proteomic analysis of human dentin.
- The identified proteins offer insights into dentin's physiological and pathological roles.
- Dentin proteins hold significant potential for in vivo analysis and tissue engineering applications in dental pulp regeneration.
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